Rishikesh K Tiwari
University of Allahabad, Allahabad, India
Title: Pesticides (chlorpyrifos, cypermethrin and their combination) induced Oxidative stress in stinging catfish, Heteropneustes fossilis
Biography
Biography: Rishikesh K Tiwari
Abstract
Pesticides contributed greatly to pest control and agricultural output whose remarkable proportion reaches to aquatic system by runoff. This results in contamination of water bodies and causes serious health problems to non- target organisms including fish. It also affects the human health by biomagnifications. Therefore, present study was carried out to investigate the sub-lethal effect of chlorpyrifos (organophosphate) cypermethrin (pyrethroid) and their combination to explore their impact on stress parameters such as oxidative stress enzymes (Super Oxide Dismutase; SOD), (Catalase; CAT), (Glutathione-S-Transferase; GST) and antioxidant indices such as (Glutathione Reduced; GSH) and (Lipid peroxidation; LPO) in a stinging catfish Heteropneustes fossiis. Fishes were procured, acclimatised under laboratory conditions and were fed with commercially available fish pellets ad libitum. To study the effect of chlorpyrifos, cypermethrin and their combination, fishes were exposed to 5% & 10% of their LC50 doses up to 96h. After exposure, the fishes were sacrificed and the tissues (brain, liver, kidney, gills and muscles) and blood were collected for estimations. A significant dose-dependent changes (p<0.05) in level of anti-oxidant stress parameters were observed in exposed group as compared to control. This preliminary study reflects the effect of these pesticides which serves as the potential biomarker of pesticides contamination in aquatic system.