Zuyue Sun
Shanghai Institute of Planned Parenthood Research, China
Title: A newly-developed analytical method for curdione and its application in concomitant toxicokinetics of reproductive toxicity studies
Biography
Biography: Zuyue Sun
Abstract
Objective: The objective of this study was to determine the toxicokinetic profile of curdione in pregnant SD rats as well as the transference of curdione into the fetus through the placental barrier system using LC-MS/MS.
Methods: A sensitive analytical method for determining the plasma concentration of curdione was developed and applied in the determination of curdione in pregnant SD rats as a simulated model. Thirteen pregnant SD rats were treated with 7, 21 and 63 mg/kg curdione once daily from GD6 to GD15. Blood samples were collected at different time points on GD6 and GD15. Maternal plasma, placental plasma, placenta tissue, amniotic fluid and fetal tissue were collected for concentration analysis after all the animals were sacrificed following one repeated dose.
Results: The results indicated that Cmax, AUC(0-t) and AUC(0-∞) increased in a dose-dependent manner both on GD6 and GD15. At 7 mg/kg group, the total serum clearance value on GD15 was reduced to approximately 16.4% of that on GD6, and the volume of distribution was also significantly decreased (p<0.05). Curdione could be detected in the maternal plasma, placental plasma, placenta tissue, amniotic fluid and fetal tissue, and its concentration in the fetal tissue reached saturation at 21 mg/kg. Conclusion: It presents with the risk of toxic accumulation in the concomitant toxicokinetics of reproductive toxicity studies in SD rats and may affect the fetus via transference through the placental barrier system.